Cloned (Comment) | Organism |
---|---|
generating of transgenic rice lines overexpressing the OsIRL gene under an abscisic acid inducible promoter, the OsIRL transgenic rice line activated by abscisic acid treatment is tolerant against methyl viologen and glucose/glucose oxidase-induced stress in rice leaves and suspension-cultured cells, cloning of recombinant enzyme as six-His tagged OsIRL protein for antibody production | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-hydroxyformononetin + NADPH + H+ | Oryza sativa | key enzyme in isoflavonoid phytoalexin biosynthesis | vestitone + NADP+ | - |
? | |
additional information | Oryza sativa | involvement of OsIRL in homeostasis of reactive oxygen species, products of OsIRL are positive regulators to suppress cell death under oxidative stress | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Oryza sativa | Q8VYH7 | L. cv. Jinheung | - |
Purification (Comment) | Organism |
---|---|
as six-His tagged OsIRL protein, the generated recombinant OsIRL protein is used for antibody preparation | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | - |
Oryza sativa | - |
cell suspension culture | - |
Oryza sativa | - |
root | - |
Oryza sativa | - |
seedling | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-hydroxyformononetin + NADPH + H+ | key enzyme in isoflavonoid phytoalexin biosynthesis | Oryza sativa | vestitone + NADP+ | - |
? | |
additional information | involvement of OsIRL in homeostasis of reactive oxygen species, products of OsIRL are positive regulators to suppress cell death under oxidative stress | Oryza sativa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IFR | - |
Oryza sativa |
isoflavone reductase | - |
Oryza sativa |
OsIRL | - |
Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Oryza sativa |
Organism | Comment | Expression |
---|---|---|
Oryza sativa | After embedding of seeds in agar containing 2',7',-dichlorofluorescin, the H2O2 is highly stimulated in the elongation region compared with the root apex. The OsIRL mRNA expression level in 3-day-old roots is weakly expressed in the apex region, it shows an increment accompanying the increase in H2O2 concentration, results indicate that the expression level and localization of OsIRL is associated with H2O2 accumulation. | down |
Oryza sativa | OsIRL gene and protein are downregulated in young seedling roots treated with diphenyleneiodonium, known quencher of reactive oxygen species, but to a lesser extent when compared with glutathione treatment in roots | down |
Oryza sativa | OsIRL gene and protein are downregulated in young seedling roots treated with reduced glutathione, a known quencher of reactive oxygen species, in roots of 3 day-old young seedlings grown on glutathione, reactive oxygen species levels are significantly decreased by approximately 50% in the presence of 1 mM glutathione compared with untreated controls | down |
Oryza sativa | OsIRL gene and protein are shown to be downregulated in young seedling roots treated with reduced glutathione and diphenyleneiodonium | down |
Oryza sativa | the expression of OsIRL is slightly increased after treatment 0.02 mM abscisic acid in wild-type calli, suggesting a possible reason for glucose/glucose oxidase stress resistance | up |
Oryza sativa | the OsIRL transcript level in rice suspension-cultured cells is induced by FeCl3 within 4 h after treatment, but down-regulated when co-treated with glutathione | up |
Oryza sativa | the OsIRL transcript level in rice suspension-cultured cells is induced by glucose/glucose oxidase within 4 h after treatment, but down-regulated when co-treated with glutathione | up |
Oryza sativa | the OsIRL transcript level in rice suspension-cultured cells is induced by methyl viologen within 4 h after treatment, but down-regulated when co-treated with glutathione | up |
Oryza sativa | the OsIRL transcript level in rice suspension-cultured cells is induced by the oxidant H2O2, but down-regulated when co-treated with glutathione | up |
Oryza sativa | OsIRL transcript level in rice suspension-cultured cells is found to be by hydrogen peroxide, ferric chloride, methyl viologen and glucose/glucose oxidase | up |
General Information | Comment | Organism |
---|---|---|
malfunction | transgenic rice lines overexpressing the OsIRL gene under an abscisic acid inducible promoter are tolerant against methyl viologen (MV) and glucose/glucose oxidase-induced stress in rice leave and suspension-cultured cells | Oryza sativa |